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Current eukaryotic LSU rRNA primers


Reference organism: Saccharomyces cerevisiae
Number of nucleotides: 3550

        10        20        30        40        50
....|....|....|....|....|....|....|....|....|....|
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC
                              |----NLF31/19-----> 
                                                  
                                                  
                                                  

        60        70        80        90       100
....|....|....|....|....|....|....|....|....|....|
GAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCT
                                        <---NLR108
                                                  
                                                  
                                                  

       110       120       130       140       150
....|....|....|....|....|....|....|....|....|....|
TTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAG
/18----|                                          
                                                  
                                                  
                                                  

       160       170       180       190       200
....|....|....|....|....|....|....|....|....|....|
CGTCATTTGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGC
                                 |-----NLF184/21--
                                 <-----NLR204/21--
                                                  
                                                  

       210       220       230       240       250
....|....|....|....|....|....|....|....|....|....|
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGATTGCCTTAGTAACGGC
--->                                              
---|                                              
                                                  
                                                  

       260       270       280       290       300
....|....|....|....|....|....|....|....|....|....|
GAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCG
                                                  
                                                  
                                                  
                                                  

       310       320       330       340       350
....|....|....|....|....|....|....|....|....|....|
AGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCC
                                                  
                                                  
                                                  
                                                  

       360       370       380       390       400
....|....|....|....|....|....|....|....|....|....|
TTGGAACAGGACGTCATAGAGGGTGAGCATCCCGTGTGGCGAGGAGTGCG
                                                  
                                                  
                                                  
                                                  

       410       420       430       440       450
....|....|....|....|....|....|....|....|....|....|
GTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTC
                                                  
                                                  
                                                  
                                                  

       460       470       480       490       500
....|....|....|....|....|....|....|....|....|....|
TAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGAT
                                              |--N
                                              <--N
                                                  
                                                  

       510       520       530       540       550
....|....|....|....|....|....|....|....|....|....|
AGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGT
LF497/16--->                                      
LR512/16---|                                      
                                                  
                                                  

       560       570       580       590       600
....|....|....|....|....|....|....|....|....|....|
GAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGG
                                                  
                                                  
                                                  
                                                  

       610       620       630       640       650
....|....|....|....|....|....|....|....|....|....|
TGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTG
                                                  
                                                  
                                                  
                                                  

       660       670       680       690       700
....|....|....|....|....|....|....|....|....|....|
GGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGC
           |--NLF662/16--->                       
           <--NLR677/16---|                       
                                                  
                                                  

       710       720       730       740       750
....|....|....|....|....|....|....|....|....|....|
CTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGAC
                                                  
                                                  
                                                  
                                                  

       760       770       780       790       800
....|....|....|....|....|....|....|....|....|....|
TGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGCCCGTCTT
                                           |------
                                             |----
                                             <----
                                           <------

       810       820       830       840       850
....|....|....|....|....|....|....|....|....|....|
GAAACACGGACCAAGGAGTCTAACGTCTATGCGAGTGTTTGGGTGTAAAA
-NLF794/25------->                                
-NLF796/21----->                                  
-NLR816/21-----|                                  
-NLR818/25-------|                                

       860       870       880       890       900
....|....|....|....|....|....|....|....|....|....|
CCCATACGCGTAATGAAAGTGAACGTAGGTTGGGGCCTCGCAAGAGGTGC
                                                  
                                                  
                                                  
                                                  

       910       920       930       940       950
....|....|....|....|....|....|....|....|....|....|
ACAATCGACCGATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCATAGC
                                                  
                                                  
                                                  
                                                  

       960       970       980       990      1000
....|....|....|....|....|....|....|....|....|....|
TGTTGGGACCCGAAAGATGGTGAACTATGCCTGAATAGGGTGAAGCCAGA
                                                  
                                                  
                                                  
                                                  

      1010      1020      1030      1040      1050
....|....|....|....|....|....|....|....|....|....|
GGAAACTCTGGTGGAGGCTCGTAGCGGTTCTGACGTGCAAATCGATCGTC
                                                  
                                                  
                                                  
                                                  

      1060      1070      1080      1090      1100
....|....|....|....|....|....|....|....|....|....|
GAATTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCTGGTT
                                                  
                                                  
                                                  
                                                  

      1110      1120      1130      1140      1150
....|....|....|....|....|....|....|....|....|....|
CCTGCCGAAGTTTCCCTCAGGATAGCAGAAGCTCGTATCAGTTTTATGAG
    |-----NLF1105/22----->                        
    <-----NLR1126/22-----|                        
                                                  
                                                  

      1160      1170      1180      1190      1200
....|....|....|....|....|....|....|....|....|....|
GTAAAGCGAATGATTAGAGGTTCCGGGGTCGAAATGACCTTGACCTATTC
                                                  
                                                  
                                                  
                                                  

      1210      1220      1230      1240      1250
....|....|....|....|....|....|....|....|....|....|
TCAAACTTTAAATATGTAAGAAGTCCTTGTTACTTAATTGAACGTGGACA
                                                  
                                                  
                                                  
                                                  

      1260      1270      1280      1290      1300
....|....|....|....|....|....|....|....|....|....|
TTTGAATGAAGAGCTTTTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGA
                                                  
                                                  
                                                  
                                                  

      1310      1320      1330      1340      1350
....|....|....|....|....|....|....|....|....|....|
TGCGGGATGAACCGAACGTAGAGTTAAGGTGCCGGAATACACGCTCATCA
                                                  
                                                  
                                                  
                                                  

      1360      1370      1380      1390      1400
....|....|....|....|....|....|....|....|....|....|
GACACCACAAAAGGTGTTAGTTCATCTAGACAGCCGGACGGTGGCCATGG
                                                  
                                                  
                                                  
                                                  

      1410      1420      1430      1440      1450
....|....|....|....|....|....|....|....|....|....|
AAGTCGGAATCCGCTAAGGAGTGTGTAACAACTCACCGGCCGAATGAACT
         |-----NLF1410/23------>                  
         <-----NLR1432/23------|                  
                                                  
                                                  

      1460      1470      1480      1490      1500
....|....|....|....|....|....|....|....|....|....|
AGCCCTGAAAATGGATGGCGCTCAAGCGTGTTACCTATACTCTACCGTCA
                                                  
                                                  
                                                  
                                                  

      1510      1520      1530      1540      1550
....|....|....|....|....|....|....|....|....|....|
GGGTTGATATGATGCCCTGACGAGTAGGCAGGCGTGGAGGTCAGTGACGA
                                                  
                                                  
                                                  
                                                  

      1560      1570      1580      1590      1600
....|....|....|....|....|....|....|....|....|....|
AGCCTAGACCGTAAGGTCGGGTCGAACGGCCTCTAGTGCAGATCTTGGTG
                                                  
                                                  
                                                  
                                                  

      1610      1620      1630      1640      1650
....|....|....|....|....|....|....|....|....|....|
GTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGG
                                                  
                                                  
                                                  
                                                  

      1660      1670      1680      1690      1700
....|....|....|....|....|....|....|....|....|....|
TTCCACGTCAACAGCAGTTGGACGTGGGTTAGTCGATCCTAAGAGATGGG
                          |--NLF1677/17--->       
                           <--NLR1694/17---|      
                                                  
                                                  

      1710      1720      1730      1740      1750
....|....|....|....|....|....|....|....|....|....|
GAAGCTCCGTTTCAAAGGCCTGATTTTATGCAGGCCACCATCGAAAGGGA
                                                  
                                                  
                                                  
                                                  

      1760      1770      1780      1790      1800
....|....|....|....|....|....|....|....|....|....|
ATCCGGTAAGATTCCGGAACTTGGATATGGATTCTTCACGGTAACGTAAC
                                                  
                                                  
                                                  
                                                  

      1810      1820      1830      1840      1850
....|....|....|....|....|....|....|....|....|....|
TGAATGTGGAGACGTCGGCGCGAGCCCTGGGAGGAGTTATCTTTTCTTCT
                                                  
                                                  
                                                  
                                                  

      1860      1870      1880      1890      1900
....|....|....|....|....|....|....|....|....|....|
TAACAGCTTATCACCCCGGAATTGGTTTATCCGGAGATGGGGTCTTATGG
                                                  
                                                  
                                                  
                                                  

      1910      1920      1930      1940      1950
....|....|....|....|....|....|....|....|....|....|
CTGGAAGAGGCCAGCACCTTTGCTGGCTCCGGTGCGCTTGTGACGGCCCG
                                                  
                                                  
                                                  
                                                  

      1960      1970      1980      1990      2000
....|....|....|....|....|....|....|....|....|....|
TGAAAATCCACAGGAAGGAATAGTTTTCATGCTAGGTCGTACTGATAACC
                                                |-
                                                |-
                                                <-
                                                  

      2010      2020      2030      2040      2050
....|....|....|....|....|....|....|....|....|....|
GCAGCAGGTCTCCAAGGTGAACAGCCTCTAGTTGATAGAATAATGTAGAT
--NLF1999/18--->                                  
--NLF1999/19---->                                 
--NLR2016/18---|                                  
                                                  

      2060      2070      2080      2090      2100
....|....|....|....|....|....|....|....|....|....|
AAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGATAAGGATTGGCTCT
                        |------NLF2075/24------>  
                        <------NLR2098/24------|  
                                                  
                                                  

      2110      2120      2130      2140      2150
....|....|....|....|....|....|....|....|....|....|
AAGGGTCGGGTAGTGAGGGCCTTGGTCAGACGCAGCGGGCGTGCTTGTGG
                                                  
                                                  
                                                  
                                                  

      2160      2170      2180      2190      2200
....|....|....|....|....|....|....|....|....|....|
ACTGCTTGGTGGGGCTTGCTCTGCTAGGCGGACTACTTGCGTGCCTTGTT
                                                  
                                                  
                                                  
                                                  

      2210      2220      2230      2240      2250
....|....|....|....|....|....|....|....|....|....|
GTAGACGGCCTTGGTAGGTCTCTTGTAGACCGTCGCTTGCTACAATTAAC
                                                  
                                                  
                                                  
                                                  

      2260      2270      2280      2290      2300
....|....|....|....|....|....|....|....|....|....|
AGATCAACTTAGAACTGGTACGGACAAGGGGAATCTGACTGTCTAATTAA
                                                  
                                                  
                                                  
                                                  

      2310      2320      2330      2340      2350
....|....|....|....|....|....|....|....|....|....|
AACATAGCATTGCGATGGTCAGAAAGTGATGTTGACGCAATGTGATTTCT
                                          |-------
                                           |----NL
                                          <----NLR
                                          <-------

      2360      2370      2380      2390      2400
....|....|....|....|....|....|....|....|....|....|
GCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGAGTAA
NLF2343/26------->                                
F2344/20---->                                     
2362/20----|                                      
NLR2368/26-------|                                

      2410      2420      2430      2440      2450
....|....|....|....|....|....|....|....|....|....|
ACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATC
                                                  
                                                  
                                                  
                                                  

      2460      2470      2480      2490      2500
....|....|....|....|....|....|....|....|....|....|
TAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTCCCTATC
                                                  
                                                  
                                                  
                                                  

      2510      2520      2530      2540      2550
....|....|....|....|....|....|....|....|....|....|
TACTATCTAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAGCG
                                                  
                                                  
                                                  
                                                  

      2560      2570      2580      2590      2600
....|....|....|....|....|....|....|....|....|....|
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTTTGACATTGTGAAGAGA
|----NLF2551/21----->                             
<----NLR2571/21-----|                             
                                                  
                                                  

      2610      2620      2630      2640      2650
....|....|....|....|....|....|....|....|....|....|
CATAGAGGGTGTAGAATAAGTGGGAGCTTCGGCGCCAGTGAAATACCACT
                                                  
                                                  
                                                  
                                                  

      2660      2670      2680      2690      2700
....|....|....|....|....|....|....|....|....|....|
ACCTTTATAGTTTCTTTACTTATTCAATGAAGCGGAGCTGGAATTCATTT
                                                  
                                                  
                                                  
                                                  

      2710      2720      2730      2740      2750
....|....|....|....|....|....|....|....|....|....|
TCCACGTTCTAGCATTCAAGGTCCCATTCGGGGCTGATCCGGGTTGAAGA
                                                  
                                                  
                                                  
                                                  

      2760      2770      2780      2790      2800
....|....|....|....|....|....|....|....|....|....|
CATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAAACGATAA
            |---NLF2763/19---->                   
            <---NLR2781/19----|                   
                                                  
                                                  

      2810      2820      2830      2840      2850
....|....|....|....|....|....|....|....|....|....|
CGCAGATGTCCTAAGGGGGGCTCATGGAGAACAGAAATCTCCAGTAGAAC
                                                  
                                                  
                                                  
                                                  

      2860      2870      2880      2890      2900
....|....|....|....|....|....|....|....|....|....|
AAAAGGGTAAAGCCCCTTAGTTTGATTTCAGTGTGAATACAAACCATTGA
                                                  
                                                  
                                                  
                                                  

      2910      2920      2930      2940      2950
....|....|....|....|....|....|....|....|....|....|
AAGTGTGGCCTATCGATCCTTTAGTCCCTCGGAATTTGAGGCTAGAGGTG
                                                  
                                                  
                                                  
                                                  

      2960      2970      2980      2990      3000
....|....|....|....|....|....|....|....|....|....|
CCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGTCAAGCGTTCAT
                                                  
                                                  
                                                  
                                                  

      3010      3020      3030      3040      3050
....|....|....|....|....|....|....|....|....|....|
AGCGACATTGCTTTTTGATTCTTCGATGTCGGCTCTTCCTATCATACCGA
         |------NLF3010/24------>                 
         <------NLR3033/24------|                 
                                                  
                                                  

      3060      3070      3080      3090      3100
....|....|....|....|....|....|....|....|....|....|
AGCAGAATTCGGTAAGCGTTGGATTGTTCACCCACTAATAGGGAACATGA
                                       |------NLF3
                                       <------NLR3
                                                  
                                                  

      3110      3120      3130      3140      3150
....|....|....|....|....|....|....|....|....|....|
GCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGAAT
090/24------>                                     
113/24------|                                     
                                                  
                                                  

      3160      3170      3180      3190      3200
....|....|....|....|....|....|....|....|....|....|
GTTACCAGCAATAGTAATTGAACTTAGTACGAGAGGAACAGTTCATTCGG
                                                  
                                                  
                                                  
                                                  

      3210      3220      3230      3240      3250
....|....|....|....|....|....|....|....|....|....|
ATAATTGGTTTTTGCGGCTGTCTGATCAGGCATTGCCGCGAAGCACCATC
                                                  
                                                  
                                                  
                                                  

      3260      3270      3280      3290      3300
....|....|....|....|....|....|....|....|....|....|
CGCTGGATTATGGCTGAACGCCTCTAAGTCAGAATCCATGCTAGAACGCG
             |----NLF3264/21----->                
             <----NLR3284/21-----|                
                                                  
                                                  

      3310      3320      3330      3340      3350
....|....|....|....|....|....|....|....|....|....|
GTGATTTCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCCTT
                                                  
                                                  
                                                  
                                                  

      3360      3370      3380      3390      3400
....|....|....|....|....|....|....|....|....|....|
GTGGCGTCGCTGAACCATAGCAGGCTAGCAACGGTGCACTTGGCGGAAAG
                                                  
                                                  
                                                  
                                                  

      3410      3420      3430      3440      3450
....|....|....|....|....|....|....|....|....|....|
GCCTTGGGTGCTTGCTGGCGAATTGCAATGTCATTTTGCGTGGGGATAAA
                                                  
                                                  
                                                  
                                                  

      3460      3470      3480      3490      3500
....|....|....|....|....|....|....|....|....|....|
TCATTTGTATACGACTTAGATGTACAACGGGGTATTGTAAGCGGTAGAGT
                                                  
                                                  
                                                  
                                                  

      3510      3520      3530      3540         
....|....|....|....|....|....|....|....|....|....
AGCCTTGTTGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTG
             <-----NLR3535/22-----|              
                                                 
                                                 
                                                 

Document last updated: Tue Jul 15 12:00:55 MEST 2003