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NLF1999/18

Forward primer
Sequence: 5´ - CCGCAKCAGGTCTCCAA - 3´
Base composition: A: 4.0 C: 7.0 G: 3.5 T: 2.5
Length: 18 nucleotides
%GC: 61.8 %
Melting Temperature: 55.0 °C
Corresponding position in Saccharomyces cerevisiae: 1999 - 2016
Superposition of forward primers on a secondary structure model of Saccharomyces cerevisiae
Use: PCR + Sequencing
Comments: We have had more success with the longer primer NLF1999/19
Published in:
  • Van der Auwera G., Chapelle S. and De Wachter R. (1994) "Structure of the large ribosomal subunit RNA of Phytophtora megasperma, and phylogeny of the oomycetes" FEBS Letters 338, 133-136
    Designation of the primer in this publication: 18

Submitted by: Jan Wuyts

Document last updated: Tue Jul 15 12:00:52 MEST 2003